Figure 3.

Detailed analysis of the bleach profiles during the measurement of PBsome mobility in the red alga P. cruentum. A, Representative image taken 8 s after bleaching. Areas (zones) with different distances from the central zone (A) are labeled by yellow rectangles as B (0.5 μm), C (1.1 μm), and D (1.5 μm). B, Typical dynamics of a one-dimensional bleach profile taken at characteristic times after bleaching (as labeled). Curves were constructed based on two-dimensional images at different time during the FRAP routine (Fig. 1) by averaging of two-dimensional data across x axes (the red curve 8 s represents averaging across the red rectangle in A). C, Dynamic changes in average values of the A to D zones presented in A during the FRAP routine (Fig. 1). r.u., Relative units.