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. 2014 Jun 24;165(4):1632–1646. doi: 10.1104/pp.114.243741

Figure 4.

Figure 4.

Immunoblots with antibodies against essential subunits of the photosynthetic protein complexes of wild-type (WT) tobacco and PetC and PetM transformants before and at different time points after RNAi induction. Isolated thylakoid membranes were used, and equal amounts of chlorophyll were loaded. For approximate quantification, wild-type samples harvested before RNAi induction were diluted to 25% and 50%, respectively. Accumulation of PSII was probed with antibodies against PsbD and PsbE. Accumulation of the cyt-bf was probed with antibodies against PetA, PetB, and PetC. Accumulation of PSI was probed with antibodies against the reaction center subunits PsaA and PsaB. ATP synthase accumulation was probed with an antibody against AtpB. A, Protein accumulation in wild-type tobacco and the two PetM RNAi transformants. B, Protein accumulation in wild-type tobacco and the two PetC RNAi transformants.