Figure 4.

Expression analysis of selected transcription factors in wild-type, tga256, and ora59 plants. Four-week-old soil-grown wild-type (WT), tga256, and ora59 plants were sprayed with 1 mm ACC or 1 mm ACC and 1 mm SA and harvested after 24 h. The control values are from plants sprayed for 24 h with water. The relative transcript levels of the indicated transcription factors were determined by quantitative RT-PCR analysis. The relative expression (Rel. expression) values in wild-type plants after 24 h after ACC treatment were set to 100%. The mean values (± se) of four to five biological replicates are shown.