Effect of SlTIP2;2 overexpression on TYLCV infection of tomato and Arabidopsis. A, Appearance of symptoms in transgenic tomato plants overexpressing the tomato gene TIP2;2 [Tom-TIP2;2(i)] and in nontransgenic control plants [Tom-wt(i)] at 30 dpi. Note that the transgenic tomato grew better than the wild type and exhibited less severe symptoms. B to D, qPCR estimations of relative amounts of TYLCV in tomato and Arabidopsis plants at 7 dpi. Tomato β-actin and Arabidopsis UBQ10 were used as calibrating genes. B, Tom-TIP2;2(i), White bar; Tom-wt(i) plants, black bar. C, Col-SlTIP2;2(i), White bar; Col-wt(i), black bar. D, Col-wt(i), White bar; mutant tip2;2, black bar. Asterisk indicates a significant difference (Student’s t test, P < 0.05). Data points are means ± se (n = 5–10). [See online article for color version of this figure.]