Figure 3.

Rapid and reversible response of the small phyB-NBs to changes in irradiance or R:FR. Arabidopsis plants were grown under high-irradiance white light for 2 weeks. The number of phyB-NBs per nucleus was recorded under high irradiance and high R:FR for 25 min (white background), transferred to low irradiance or low R:FR for 65 min (gray background), and returned to high irradiance and high R:FR for an additional 60 min (white background). Confocal images were recorded from different plants every 4 min in three independent experiments. Data show the moving average of three successive time points ± se. The dotted lines show the number of phyB-NBs in plants kept under high irradiance and high R:FR during the whole experiment (recorded at the end of each experiment). [See online article for color version of this figure.]