Figure 2.

Extracellular loops are dispensable for AtCASP1 localization at the CSD. Representative confocal images of AtCASP1-GFP mutant/wild type-mCherry crosses (green and red labels, respectively) are shown. Numbers indicate positions of the cell shown counted from the first elongating endodermal cell (the lower the number, the closer the cell is to the root apical meristem). White arrows point to the position of the CSD. The schematic summarizes the position of deletions and mutagenized residues that affect or do not affect localization (above and below, respectively). Mutations are annotated on a sequence logo of the CASPL1A subgroup to highlight the conservation of the mutagenized residues. Yellow bars indicate the positions of the extracellular loops. [See online article for color version of this figure.]