Figure 3. Effects of knocking down USP9X on cell cycle distribution. Cell cycle analysis of iKD-USP9X-BxPC3 cells cultured in the absence or presence of Dox (1 µg/ml) for 4 d. The Telford Reagent⁴⁶ and FACS analysis (UNMC Cell Analysis Core Facility) were used to determine cell cycle distribution. The Student t test was used to determine P values (n = 2), and error bars represent standard deviations. This experiment was repeated with similar results.