Figure 6. Effects of USP9X inhibitor, WP1130, on in vitro PDAC cell growth. BxPC3, Capan1, CD18, Hs766T, and S2-013, PDAC cells were cultured in the indicated concentrations of WP1130 for 72 h. All conditions tested included the same concentration of the DMSO vehicle. Cell viability was assessed by MTT assay, as described in the Materials and Methods. Measurements from triplicate cultures (n = 3) were averaged and plotted. Error bars represent standard deviations, and the Student t test was used to determine significance. This experiment was repeated and similar results were obtained.