Figure 3. Anti-CXCL4 mAb suppresses the induction of p53 and Bax through CXCR3 to reduce the apoptosis of intestinal epithelia by 5-FU. (A) Representative photomicrographs of apoptotic cells in the jejunum from WT, cxcr3^−/−, and p53^−/− mice. The mice were treated with 1 mg/kg anti-CXCL4 mAb 2 h before 5-FU (300 mg/kg) and sacrificed at 24 h post-5FU for the TUNEL staining of the apoptotic cells. Figure 2C (d–f) and (A) are identical pictures from the same experiment. (B) Quantitative presentation of the TUNEL-positive cells per crypt is shown in (A). Ten crypts per microscope field and 10 fields per mouse were counted for TUNEL-positive cells. (C) Photographs of the western blot analysis of the jejunum tissues from the mice described in (A) with antibodies against p53 and Bax. (D and E) Quantitative presentation of p53 and Bax is shown in (C). The p53 and Bax levels in the antibody treatment group (5-FU+anti-CXCL4 mAb) are expressed as a percent of their respective controls (5-FU). Data are presented as mean ± SD, n = 3 mice per group. *P < 0.05, **P < 0.01 vs. control group.