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. 2014 Jul 16;15(1):243. doi: 10.1186/1471-2105-15-243

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© Oliveira et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Regions with conserved RNA secondary structures and their respective melting curves. This figure corresponds to analysis of IMNV-like group sequences. (A) Indication of regions with conserved secondary structure inside RdRp coding sequences, identified using RNAz. (B) Minimum free energy models calculated using RNAfold corresponding to each conserved region identified by RNAz. Structures are colored according to base-pairing probabilities. Red color denotes the high probability and purple denotes low probability of a given base is paired or not. For unpaired regions the color denotes the probability of being unpaired. (C and D) Melting curves calculated from conserved regions using softwares RNAheat and MELTSIM respectively.