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. 2014 Jul 16;15(1):243. doi: 10.1186/1471-2105-15-243

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© Oliveira et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Differentiating members of the subgroup TVV1 using in silico melting curves. A region with variable RNA secondary structure (A) and a region with conserved RNA structure (B) were obtained from the alignment of ORF2 of all members of the group using the software RNAz. The curves were generated by RNAheat and MELTSIM to conserved regions (C) and variable regions (D). Each denaturation curve is marked with a different color: Dark blue lines to TVV1-C344; orange lines to TVV1-OC3; yellow lines to TVV1-OC4; green lines to TVV1-OC5; dark red lines to TVV1-UR1; light blue lines to TVV1-UH9 and dark green lines to TVV1_I.