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. 2014 Jul 11;46(7):e105. doi: 10.1038/emm.2014.36

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Copyright © 2014 KSBMB.

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Phosphorylation of Drp1^S616 is mediated by CDK5 in neurons. Immunohistochemical labeling signals of p35 (a) and CDK5 (b), or pDrp1^S616 (d) and CDK5 (e) in adult rat cerebral cortex. A merged image is shown in c and f. Nuclei were counterstained with Hoechst 33342 (blue). (g) Immunoblot of Drp1 and pDrp1^S616 in cultured cortical neurons (DIV10) following the treatment with different concentrations of roscovitine, PD98059 and Bisindolylmaleimide I (Bis-I) for two hours. (h) Immunocytochemical labeling of pDrp1^S616 (red) in cultured cortical neurons transfected with GFP (left panels) or DN-CDK5-GFP (right panels). Nuclei were counterstained with Hoechst 33342 (blue). Arrows indicate cells expressing GFP or DN-CDK5-GFP. (i) Immunoblot of Drp1 and pDrp1^S616 in HeLa cells transfected with or without GFP, p25-GFP and DN-CDK5. Arrows indicate the bands derived from transfected YFP-hDrp1 proteins, while arrowheads indicate the bands derived from the endogenous Drp1.