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. 2014 Jul 29;4(7):e414. doi: 10.1038/tp.2014.51

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Copyright © 2014 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Differentiation of human embryonic stem cells (hESCs) into common myeloid progenitors (CMPs). (a) Photomicrograph of a pluripotent hESC (H9) colony with smooth margins. (b) Hematopoietic stem cell (HSC)-containing colony after 8 days of coculture. (c) Flow cytometry showing CD34⁺ cells (red) isolated from colonies after 8 days of coculture, isotype control is black. (d–f) Flow cytometry dot plots of CD34 and CD45 expression in HSC cultured with ganulocyte-macrophage colony-stimulating factor after 1, 3 and 12 days. (d) The CD34^lo/CD45^hi population increased from 2–4% on day 1 to (e) 10–30% by day 3 and (f) 50–70% by day 12. (g) Example of isotype control for d–f. (h) Dot plot and (i) histogram of CD45⁺ staining in CMPs after 12 days. (j) Histogram of CD43⁺ immunostaining in CMPs after 12 days. Scale bars, 100 μm. Aβ, amyloid-β CFSE, carboxyfluorescein diacetate succinimidyl ester; DC, dendritic cell; IL, interleukin.