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. 2014 Jul 1;107(1):126–133. doi: 10.1016/j.bpj.2014.05.018

Figure 5.

Figure 5

Effects of Vm magnitude and turgor pressure on eM phase. (A) Command magnitude was varied from 20 to 80 mV peak at 98, 195, and 391 Hz. Example traces are in top panel; average (+/−se) phase (re: 20 mV response) is plotted in bottom panel. No differences in phase were found. (B) Turgor pressure was varied from 0 to 8 mm Hg via the patch pipette. Though magnitude increased with pressure, phase remains unaffected. Example traces are in top panel (low frequency responses to left of eM response were calibration movements of the image). Note that smart averaging (see Methods) was performed for each trace, hence the different noise levels. An example of frequency dependence is in bottom panel. No changes were observed. Average results for three cells at 390 Hz (mean +/− se @ 4, 6, and 8 re: 2 mm Hg condition) are -0.063 +/− 0.02, -0.055 +/− 0.04, and -0.019 +/− 0.06, respectively. All phase analysis was via FFT. Scale bar: 3 um. Chloride is 140 mM. To see this figure in color, go online.