Figure 5.

The role of translocation dynamics in regulation of Gi-mediated Ca²⁺ oscillation (in silico analysis from a single γ subunit model) (a) Simplified biochemical reaction schematic of the Gi coupled GPCR activated signaling network that induces Ca²⁺ oscillations in the presence of βγ translocation between plasma membrane and internal membranes. (b) Simulation of the time course of the Ca²⁺ oscillation pattern for different γ translocation rates (k_t). (c) Simulation of the corresponding kinetics of γ translocation to internal membrane. (d) Increasing the translocation rate leads to a decreased number of Ca²⁺ spikes measured over a fixed time period (240 s). (e) Increasing the translocation rate leads to earlier switching off and shorter duration of Ca²⁺ oscillation (black: low; red: high dose). To see this figure in color, go online.