Sedimentation velocity of FhaC at 3.3 mg/mL and FhaC-ΔH1 at 3.0 mg/mL in 1% d17-OG, 0.5 M imidazole pH 6, 100% D2O. (A, B, D, and E) Superposition of experimental and fitted sedimentation velocity profiles and their differences (top and bottom subpanels), at 280 nm (A and D), and using interference optics (B and E). (C and F) Sedimentation coefficient distributions c(s) normalized to the main protein peak value. To see this figure in color, go online.