Figure 1.

Structure and stability of consensus Ankyrin repeat proteins (CARPs) of different lengths and cap sequences. (A) Schematic and (B) ribbon diagram (2L6B) of constructs used in this study; N, R, and C repeats are in green, blue, and red, respectively. In panel B, polar and charged residues unique to N and C repeats are in space-filling representation. (Orange) Prolines; (magenta) asparagines (substituted to tryptophans). (C) Two-state fitting (solid lines) to GdnHCl unfolding transitions for CARP(Ala), and (D) fitted free energy changes (Eqs. 1 and 2) and (E) m-values. Error bars indicate 95% confidence intervals determined by bootstrapping. To see this figure in color, go online.