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. 2014 Jul 1;107(1):34–42. doi: 10.1016/j.bpj.2014.05.002

Figure 1.

Figure 1

Cells on our patterns. (a and b) NIH3T3 cells on triangular and circular fibronectin patches, respectively, with staining for actin and paxillin. (c–e) (Upper panels) Scheme for probing the activity for all the studied configurations showing the relevant parameters. A−,A+ and z−,z+ represent the efficient adhesion area (white dashed lines) and filopodia protrusion activity (frequency of probing and stabilization time of protrusions on A±), respectively, in the + and − directions, as indicated. (Green) Cell exploration area. The parameter dp represents the average protrusion length. (Lower panels) Typical first-step motion for the different studied configurations. Time in hh:mm:ss. Scale bars: 50 μm. To see this figure in color, go online.