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. 2014 May 27;6(7):865–881. doi: 10.15252/emmm.201303675

Figure 5. RARRES3 PLA1/2 catalytic activity triggers differentiation-signaling mediators.

Figure 5

  1. Homology 3D structural model of RARRES3 (red) aligned with the structure of H-REV107 (violet) (Ren et al, 2010).
  2. The conserved catalytic residues of RARRES3 and H-REV107 showing the proposed similarity of function.
  3. Human Arachidonic Acid (AA) levels (ng/ml) measured in cell extracts of MDA-MB-231 Parental and LM2 cellular derivatives expressing different levels of RARRES3. Data are represented as the mean of three independent experiments ± SEM.
  4. Luciferase activity of 3x-AOX peroxisome proliferation response element reporter plasmid in MDA-MB-231 parental cells transiently transfected with Mock, RARRES3- and RARRES3-DEAD-expressing vectors. Activity of 3xAOX promoter was normalized to control condition and presented in arbitrary units. Data are mean of three independent experiments with ± SD.
  5. The correlation coefficient and significance of RARRES3 expression levels in ER− BC primary tumors from the MSK/EMC meta-cohort against the expression of 13 PPAR target genes represented in the U133A affymetrix array are shown, together with the p value associated with each correlation. Moreover, the risk of lung metastasis (HR) associated with the expression of each of those genes in primary tumors is also reported.
  6. Gene set enrichment analysis (GSEA) representing association of HR of lung metastasis with the PPAR target RARRES3-correlated gene set in the human breast cancer dataset (MSK/EMC expression dataset). NES-normalized enrichment score; FDR-false discovery rate; HR-hazard ratio.
  7. The correlation coefficient of RARRES3 expression levels in ER− BC primary tumors from the MSK/EMC meta-cohort against the expression of all the genes represented in the U133A affymetrix array is shown. In red, differentiation GATA transcription factors. In black, some PPAR target genes described in (E). In green, stemness gene.