Figure 6. TMED3 and SOX12 are required for efficient clonogenic behavior but only TMED3 for clone compaction.

1. Images of representative clonal colonospheres observed under Nomarski (left panels) or GFP fluorescence (right panels) illumination. Arrows indicate cells protruding from the sphere clone after interference with TMED3. Scale bar = 100 μm.
2. Quantification of sphere phenotypes by category presented as the average number of clonal spheroids per 96-well plate, plating 1 cell per well. Overall, the total number of spheroids formed per category in triplicate experiments were (28,32,30 for SIH control; 21,22,29 for shSOX12; 25,25,28 for shTMED3).