Fig. 1.

Model for the analysis of chimerism after cell transplantation. Our experimental model of C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb mice as donors of GFP⁺ bone marrow (BM) or GFP⁺lin⁻ Sca-1⁺ cells and C57Bl6/J mice as recipients (GFP⁻). Recipient animals were exposed to 9 Gy whole-body irradiation. Suspensions of GFP⁺ bone marrow cells (5 × 10⁶/ml) or GFP⁺lin⁻Sca-1⁺ cells (3 × 10⁴/ml) were transplanted by i.v. injection into recipient animals 3 hrs after irradiation. Flow cytometry analysis (FCA) of all GFP⁺ cells (%) was performed on days 30 and 70 after cell transplantation. We detected chimerism GFP⁺ cells (%)/GFP⁻ cells (%) in the peripheral blood (PB), bone marrow (BM), spleen and thymus.