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. 2014 Jan 22;18(5):852–862. doi: 10.1111/jcmm.12230

Fig. 7.

Fig. 7

UII inhibits the proliferation of cardiac side population cells (CSPs) by c-Jun N-terminal kinase (JNK)-LRP6 signalling during mechanical stretch. CSPs proliferation was determined by Cell Viability Assay. Cultured CSPs were overexpressed LRP6 (A) or pretreated with SP600125 (SP6, 5 μM; B) with or without UII (0.1 μM) during mechanical stretch (MS). CSPs were pre-treated with SP600125 or DMSO for 30 min., then subjected to MS and treated with UII for 48 hrs. CSPs were overexpressed with LRP6-pCS2 or pCS2 for 48 hrs, then subjected to MS and treated with UII for 48 hrs. Values are expressed as mean ± SEM. **P < 0.01 versus MS. #P < 0.05 versus MS plus UII. The experiment was repeated for at least three times. (C) The ratio of CSPs per mouse was analysed by fluorescence-activated cell sorting (FACS). Representative photographs are shown. (D) The ratio of CSPs per mouse was calculated by FACS. SP600125 (SP6) or DMSO were injected intraperitoneally either with 40 mg/kg bodyweight every 3 days for 2 weeks. At 4 weeks after transverse aorta constriction (TAC) or sham operation, the number of CSPs was determined. Values are expressed as mean ± SEM. Sham:n = 5; Sham+SP6:n = 6; TAC:n = 6; TAC+SP6:n = 6. Ura: urantide. *P < 0.05 versus sham mice; #P < 0.05 versus TAC mice.