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Nrf2 expression and nuclear translocation under different conditions. HK11 cells cultured on chamber slides were treated with different conditions as indicated: HG for 48 hrs, Pal for the last 6 hrs, TPEN (8 μM) and Zn (50 μM) for the last 30 hrs. Immunofluorescent staining of Nrf2 is detected by fluorescent microscope (400 × ). DAPI dying was used for nuclear staining.
