Fig. 7.

Effects of Zn deficiency on the renal infiltration of inflammatory cells and renal inflammatory. Inflammatory cells were examined by Naphthol AS-D Chloroacetate esterase staining (A). The images of this staining from each group are representatively provided for glomeruli and tubulointerstitial areas (original magnification ×400). Arrows indicate the positive cells. Positive cells for Naphthol AS-D Chloroacetate esterase staining were counted in 10 microscope visual fields for one slide of each animal of the three (B). Renal tissues from diabetic and controls with and without TPEN treatment were subject to Western blotting assay for ICAM-1 (C). Data are presented as the mean ± SD (n = 5 at least for each group). a, P < 0.05 versus Control or TPEN group; b, P < 0.05 versus DM group. DM: diabetes.