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Characterization of cultured bone-marrow-derived endothelial progenitor cells (EPCs). (A) EPCs cultured on days 1, 4 and 7. (B) EPCs were identified by DiI-acLDL, FITC-UEA-1 and DAPI staining. Cells positive for all three dyes were defined as EPCs. (C) Flow cytometry analysis of CD34 or CD45 or Flk1 expression on EPCs. (D) RT-PCR analysis of per2 mRNA expression in cultured EPCs. (F) Immunofluorescence of PER2 expression in cultured EPCs.
