Fig. 3.

Effect and mechanism of per2 on endothelial progenitor cell (EPC) proliferation, migration, tube formation and adhesion. (A) EPC proliferation, (B) migration and (C) quantification, (D) tube formation and (E) quantification, and (F) adhesion and (G) quantification were impaired with per2 knockout. And, activation of PI3K with insulin-like growth factor 1 (IGF-1) rescued the impaired proliferation of per2^−/− EPCs. (H and I) Western blot analysis of PI3K/Akt/FoxO and CXCR4 expression. (J) Co-IP for direct interaction of PER2 and CXCR4 (*P < 0.05, **P < 0.01 versus wild-type, ##P < 0.05 versus per2^−/− n = 6).