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Hydrogen attenuated irradiation-induced splenocytes apoptosis. (A and B) Irradiation-induced splenocytes apoptosis rate was quantified by Flow Cytometry at 24, 36, 48 hrs after exposure. (C) Bcl-2, Bax, caspase-3, c-caspase-3 protein expression was detected by western blot at 14 days after exposure. (D) Normalization of Bcl-2, Bax, caspase-3, c-caspase-3 expression to Tubulin. The data were expressed as means ± SEM (n = 8), *P < 0.05.
