Table 1.
Phospho-protein array analysis of TGFBI Tg versus WT islets
| Protein (Phosphorylation sites) | Phospho-protein versus total protein ratio (P/T ratio) |
P/T ratio changes | |
|---|---|---|---|
| TGFB1 Tg | WT | Tg/WT | |
| BRCA1 (Phospho-Ser1457) | 0.97 | 0.54 | 1.80 |
| DAXX (Phospho-Ser668) | 0.08 | 0.05 | 1.76 |
| VASP (Phospho-Ser157) | 0.47 | 0.27 | 1.75 |
| p27Kip1 (Phospho-Ser10) | 1.66 | 1.02 | 1.62 |
| Histone H3.1 (Phospho-Ser10) | 1.03 | 0.65 | 1.58 |
| MEF2A (Phospho-Ser408) | 2.27 | 1.48 | 1.54 |
| AKT1S1 (Phospho-Thr246) | 0.26 | 0.18 | 1.49 |
| Paxillin (Phospho-Tyr31) | 1.41 | 0.95 | 1.49 |
| BLNK (Phospho-Tyr96) | 0.22 | 0.35 | 0.63 |
| Cyclin B1 (Phospho-Ser147) | 0.49 | 0.85 | 0.58 |
| MAPKAPK2 (Phospho-Thr334) | 0.05 | 0.11 | 0.41 |
| Smad1 (Phospho-Ser465) | 0.31 | 1.08 | 0.28 |
| Calmodulin (Phospho-Thr79/Ser81) | 0.14 | 0.74 | 0.18 |
Islets from Tg and WT mice were isolated, and cultured for 4 h in F-12K serum-free medium. Islet lysates were analyzed by Full Moon BioSystem (Sunnyvale, CA, USA) phospho-protein array. Samples were run in triplicate. The names of proteins and their phosphorylation sites assessed are indicated in column 1. The ratios of mean signals of phospho-protein versus total protein (P/T ratio) are listed in columns 2 (Tg islets) and 3 (WT islets). Fold differences between the Tg ratio over the WT ratio of each protein (P/T ratio changes) were calculated and listed in the last column. Only proteins with fold differences between Tg and WT islets above 1.5 or less than 0.67 are included in the table.