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. 2014 Apr 15;23(17):4621–4638. doi: 10.1093/hmg/ddu178

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© The Author 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Nigral axonal degeneration induced by AAV2/6-mediated expression of D620N VPS35. Representative photomicrographs of substantia nigra sections processed with Gallyas silver stain, from rats injected with AAV2/6 vectors expressing human VPS35^WT and VPS35^D620N or a control vector, to reveal degenerating axons and nerve terminals (indicated in black). Scale bar: 50 μm. Images were subjected to deconvolution, color separation and filtering (circularity index ≤0.6 to remove nuclei/soma) using ImageJ to isolate black particles representative of neuritic processes and terminals for quantitation. Examples of particles analyzed are shown pseudocolored in red. Data are expressed as the number of black particles (silver-positive neurites/terminals) and bars represent the mean ± SEM (n = 6 animals/group). **P<0.01 or ***P<0.001 by one-way ANOVA with Tukey's post hoc analysis as indicated. n.s., non-significant.