Figure 1.

Schematic depicting the procedural flow for the drug screen. Carrier sapje zebrafish are bred, embryos are collected and dechorionated at 1 dpf, and are then placed into wells containing drugs from the ENZO compound library (diluted at 1/100 in E2 for a final concentration of 33 uM and 0.1% DMSO). Each pool is screened at 4 dpf by birefringence. A positive hit is considered a well with ≤2 fish (out of 20) with abnormal birefringence. Positive hits are secondarily validated by direct DNA sequencing and by testing larger numbers of embryos.