Figure 3.

Positive hits from a non-biased drug screen in the sapje zebrafish. Positive hits from the drug screen are represented as the percentage of fish with abnormal birefringence (red) compared with the percentage of fish examined with normal birefringence (blue). 0.1% DMSO was used as a negative control (25 embryos with abnormal birefringence out of 100 total screened). MG-132 was our positive control (5 out of 56 with abnormal birefringence). Six drugs were found to give ≤2/20 fish with abnormal birefringence: pergolide (1/20), aminophylline (1/20), ergotamine (0/20), fluoxetine (0/20), flunarizine (2/20) and ropinirole (0/20).