Figure 6.

Fluoxetine does not protect against mechanical stress induced injury in sapje. Embryos were treated with 0.1% DMSO or fluoxetine for 3 days. Birefringence (as a marker of membrane stability) was examined before after a significant mechanical stress (pinning of the anterior and posterior body axis). (Left column) wild-type siblings (sibs) demonstrated normal birefringence both before and after pinning. This was regardless of treatment. (Right column) untreated sapje embryos had abnormal birefringence both before and after pinning, while fluoxetine-treated sapje embryos had normal birefringence before pinning that became abnormal within 1 min after pinning.