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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Breast Cancer Res Treat. 2014 Jun 12;146(2):259–272. doi: 10.1007/s10549-014-3014-7

Fig. 3.

Fig. 3

The combination of entinostat and lapatinib enhanced drug efficacy in terms of in vitro colony formation in a soft agar assay. For visualizing or counting, colonies were stained with 200 μL of MTT (1 mg/mL) solution for 2 hours and counted using the GelCount colony-counting system according to the manufacturer's instructions. Statistical significance was evaluated by t test using GraphPad Prism software. a BT474. b SUM190. Cells (2×103) were seeded into soft agar with the indicated drug(s) and incubated for 3 weeks. Scale bars, 100 μm. Bars in the graph represent means; error bars, standard deviation. * p <0.05 combination compared with entinostat. ** p <0.01 combination compared with either control or lapatinib. Data shown are representative of 3 experiments with similar results.