Table 2.

Clinical, pathological, and molecular features of patients with ES family of tumors

No	Nested RT-PCR in PB	FISH assay	Sex	Age	Primary site	Size of primary tumor (cm)	M0/M1 status at diagnosis	Last follow-up (months/latest status)
1	Neg	Pos	F	24	Iliac bone	>10	M1	33/DOD
2	Neg	NA	M	19	Tibia	10	M0	62/AWOD
3	Neg	NA	F	20	Paraspinal	NA	M1	15/DOD
4	Neg	Pos	M	59	Scapula	6	M0	61/AWOD
5	Neg	NA	F	29	Femur	NA	M0	53/AWOD
6	Neg	Pos	F	29	Ischium	>10	M0	5/DOD
7	Pos (EWS–ERG)	Pos	M	42	Iliac bone	>10	M1	9/DOD
8	Neg	Pos	F	20	Femur	14	M1	7/DOD
9	Neg	Pos	F	25	Iliac bone	>10	M1	55/AWD
10	Neg	Pos	M	33	Femur	7	M0	50/AWOD
11	Neg	NA	M	37	Chest wall	15	M0	49/AWOD
12	Neg	Pos	F	40	Tibia	>10	M0	7/DOD
13	Neg	NA	M	24	Chest wall	>10	M1	4/DOD
14	Neg	NA	F	19	Femur	>10	M0	1/AWD
15	Neg	Pos	F	19	Scapula	9.5	M0	44/AWOD
16	Neg	Pos	M	22	Femur	11	M1	6/DOD
17	Neg	NA	F	33	Forearm	7	M0	43/AWOD
18	Neg	NA	M	20	Humerus	NA	NA	NA/AWD
19	Neg	Pos	M	25	Fibula	10	M0	15/DOD
20	Neg	Pos	M	27	Costa	NA	M0	34/DOD
21	Neg	NA	F	37	Paraspinal	12	M0	14/DOD
22	Neg	NA	F	30	Thigh	8.5	M1	25/DOD
23	Neg	NA	F	30	Femur	NA	M0	37/AWD
24	Neg	NA	F	24	Retroperitoneal space	NA	M0	37/AWD
25	Neg	Pos	F	25	Forearm	9.5	M0	33/AWOD
26	Neg	NA	F	34	Humerus	NA	M0	34/AWD
27	Neg	NA	F	27	Pelvis	NA	M1	14/DOD
28	Neg	Pos	M	41	Abdominal wall	7	M1	34/AWD
29	Pos (EWS–FLI1 type 2)	Pos	F	23	Pelvis	NA	M1	3/DOD
30	Neg	NA	M	43	Femur	NA	M0	10/DOD
31	Neg	NA	M	20	Ribs	17	M0	28/AWOD
32	Neg	Pos	F	29	Mediastinum	15	M0	111/AWD
33	Pos (EWS–FLI1 type 1)	NA	F	21	Ischium	8	M1	18/DOD
34	Neg	NA	M	35	Femur	13	M0	26/AWOD

RT-PCR reverse transcription polymerase chain reaction, FISH fluorescent in situ hybridization, M0 no evidence of metastasis, M1 evidence of metastasis, Pos positive, Neg negative, NA not available, DOD dead of disease, AWD alive with disease, AWOD alive without disease