Figure 3.

IFN-γ responses induced by PPD-A, PPD-B, C1, and C2 in naturally Map-infected cattle (n = 17). The cocktails were tested at a concentration of 55 μg/mL per constitutive protein. PPD-A and PPD-B were assessed with concentration of 50 μg/mL. Aliquots of 25 μL of each antigenic preparation were added to 200 μL of blood samples. The results for each animal are represented by different figures and the horizontal line provides the mean of the ODIs. The dashed line represents the cutoff values used for positivity.