Figure 4.

DTH response induced by PPD-B, C1, and C2 in experimentally M. bovis-infected cattle to 90 dpi (n = 6). The cocktails were prepared with 10 μg of each antigen per 0.1 mL in PBS and PPD-B 100 μg per 0.1 mL. The animals were intradermally injected with 0.1 mL of C1, C2, or PPD-B (1 mg/mL) and the thickness of the caudal fold tuberculin skin test was measured using callipers prior and 72 h after injection. Animal responses were represented by boxes and the horizontal line provides the median with standard errors. The statistical difference between responses was determined by using Kruskall Wallis test (**P < 0.01).