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IFN-γ responses induced by PPD-B fractions in experimentally M. bovis-infected cattle (n = 6). PPD-B fractions were tested at a concentration of 36 μg/mL. Aliquots of 25 μL of each fraction were added to 200 μL of blood samples. The results for each animal are represented by different vertical lines. The fractions were first tested with blood of animals from 0 and 20 dpi. The fractions that displayed the more stimulant responses were tested again at 30, 60, and 90 dpi.
