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. 2014 Jul 8;2014:140829. doi: 10.1155/2014/140829

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Copyright © 2014 María Laura Mon et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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IFN-γ responses induced by PPD-A, PPD-B, C1, C2, C1 plus FixB and C1 plus CFP2 in cattle from a free TBB and PTB herd (n = 10). The cocktails were tested at a concentration of 55 μg/mL per constitutive protein, PPD-A and PPD-B with concentration of 50 μg/mL. Aliquots of 25 μL of each antigenic preparation were added to 200 μL of blood samples. The results for each animal are represented by different markers and the horizontal line provides the mean of the ODIs. The dashed line represents the cutoff values used for positivity.