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Effects of EM on ALP activity and osteogenic gene expression in MC3T3 cells under physiological condition. (a) MC3T3 cells were cultured in 24-well plate at 2 × 105 cells/well. Cells were treated with EM (0–10 μg/mL) for 14 days before ALP activity assay. Experiments repeated three times, each with duplicate samples. (b) Gene expression of MC3T3 cells 7 days after culture. Values are means ± SEM of genes measured for triplicate, and the experiments repeated three times.
