Figure 3. The SHH1 SAWADEE domain recognizes H3K9 methylation and adopts a unique tandem Tudor domain-like fold.
a and b, ITC-based measurements of the SAWADEE domain binding to the modified or unmodified histone peptides as indicated. Kd values are listed. NDB means no detectable binding. c, The overall structure of the SHH1 SAWADEE domain in the free form. The zinc-binding motif is shown as an enlarged ball-and-stick model, highlighting the details of the metal coordination. A bound detergent molecule 4-Cyclohexyl-1-Butyl-β-D-Maltoside moiety from the crystallization condition is shown in a stick representation.