Table 2.

Disaccharide compositional analysis of heparin samples following N-acetylation.

Samples		Unlabeled disaccharides (%)					D-labeled disaccharides (%)			Total D-labeled disaccharides (%)
	0S	2S/6S	2S6S	NS	NS2S/ NS6S	Tri S	0S	2S/6S	2S6S
CNDS	9.91	13.9	5.90	68.50	0.36	1.38	0.04	< 0.01	0.02	0.06
Ac-CNDS	3.85	14.0	81.7	0.18	0.01	0.23	< 0.01	< 0.01	0.02	0.02
Ac-d3-CNDS	2.00	5.14	2.30	0.17	< 0.01	0.25	1.69	10.3	78.2	90.1
Heparin (n=3)	3.98 ±0.42	4.97 ±0.78	4.44 ±0.43	7.40 ±1.15	9.20 ±0.98	69.7 ±1.28	0.07 ±0.01	0.04 ±0.01	0.16 ±0.01	0.27 ±0.02
65°C-48hr	4.65	6.23	4.79	11.19	0.28	72.56	0.08	0.04	0.17	0.30
85°C-48hr	5.12	6.39	5.19	10.39	0.21	72.05	0.10	0.07	0.47	0.64
121°C-0.5hr	4.18	4.61	4.18	8.36	8.08	70.09	0.08	0.06	0.35	0.49
121°C-1hr	4.22	4.99	4.51	7.05	9.61	68.79	0.08	0.08	0.67	0.83
121°C-2hr	4.01	4.40	4.71	9.55	8.80	64.94	0.15	0.25	3.19	3.58

The IC₅₀ values determined in competitive AT binding measured by SPR (See Fig. S5) are heparin 1.38 U/mL, 65°C- 48 h 1.52 U/mL, 85°C- 48 h 1.76 U/mL, 121°C- 0.5 h 1.84 U/mL, 121°C- 1 h 1.96 U/mL, and 121°C- 2 h 3.81 U/mL.