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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Immunotoxicol. 2014 Feb 3;12(1):64–73. doi: 10.3109/1547691X.2014.882449

Figure 4. Total lung cells and lung neutrophils following repetitive ODE treatments and 1 wk recovery in SRA KO mice.

Figure 4

Mice were intra-nasally treated with saline or ODE daily for 3 wk (repetitive ODE) or treated daily for 3 wk followed by no treatment for 1 wk (recovery ODE) whereupon mice were euthanized, lavage fluid removed, pulmonary vasculature perfused, and lung cells dissociated. (A) Mean total lung cells as determined by hemocytometer. (B) Mean total neutrophils (% LY6G+ neutrophils (see Supplemental Figure 1) of CD45+ cells [as determined by flow cytometry] multiplied by total lung cells) in WT and SRA KO mice. Error bars represent SE. Statistical significance (*p < 0.01,**p < 0.01, ***p < 0.001) vs. saline. ###p < 0.001: significant difference between WT repetitive ODE and WT recovery ODE (1-way ANOVA with Tukey’s post-hoc test). N = 5–8 mice/group.