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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Nature. 2014 Jun 5;510(7503):115–120. doi: 10.1038/nature13413

Figure 4. miR-34/449 deficiency causes defective ciliogenesis in the Xenopus embryonic epidermis.

Figure 4

a. MCCs in miR-34/449 morphants show reduced cilia length and number, demonstrated by immunofluorescence for Ac-α-tub (cilia) and phalloidin-488 (Actin). b. Quantification of MCC ciliation in (a). χ2-test. c. Co-staining of Ac-α-tub (cilia) and γ-tub (basal bodies) in miR-34/449 morphants reveals uneven/aggregated distribution of basal bodies, which frequently fail to form cilia. Embryos/cells analyzed: Uninjected (4/14), miR-34/449 MOs (5/30). Embryos were derived from at least two females and independent fertilizations per experiment. Error bar, s.e.m.