Table 1.
Oligonucleotide primers
| Designation | Forward and reverse sequences(5′-3′) |
|---|---|
| Primers used for GATA-2 sequencing | |
| GATA-2 exon 2 | GTTTTGAGCCTTGGGCTTT |
| CAATTTTTCAGCAGCTCGATT | |
| GATA-2 exon 3 | GGAGTCGTGATCTCAATGTCTG |
| ATCTGCTGGGGGCTATTAGAG | |
| GATA-2 exon 4 | ACTCCCTCCCGAGAACTTG |
| CGTCTGCATTTGAAGGAGTTT | |
| GATA-2 exon 5 | GAGATTTAGCCCTCCTTGACTG |
| AGCACAAAGCGCAGAGGT | |
| GATA-2 exon 6 | GAAGGTCGGGCACAATTC |
| ACAGGTGCCATGTGTCCA | |
| Primers used for validation sequencing | |
| EZH2 | CATCAAAAGTAACACATGGAAACC |
| GCTGCTTTAAAACATAATTCCACA | |
| HECW2 | GTCCATATCCTACCTCCAGTAGC |
| GACAGCTCCTGCAATGAGAGT | |
| GATA-1 | TAGACCTTGGGCAGCTCCT |
| CCTTGGTAGAGATGGGCAGTA | |
| Primers used for quantitative ChIP | |
| GATA-2 −2.4 kb | GTGGAGCTCTAGGGTACCATTT |
| TGAGGACACCTCATTAGAGCAG | |
| GATA-2 −3.5 kb | GTCCGGGGTAATTTTTCATCT |
| GCAGATAACGACTGGCTATTCA | |
| GATA-2 −4.6 kb | GAGATGAGCTAATCCCGCCGTA |
| AAGGCTGTATTTTTCCAGGCT | |
| NECDIN promoter | GAAGAGCTCCTGGACGCAGA |
| TGCAAAGTTAGGGTCGCTCAG | |