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. 2014 Jul 8;111(3):486–496. doi: 10.1038/bjc.2014.152

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Copyright © 2014 Cancer Research UK

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Self-renewal markers in isolated SP cells. Confocal analysis showed a set (Shh, Oct3/4 and β-catenin) of self-renewal marker expression in SW1990-SP cells. The first panel showed the increased expression of Shh (red) along with PD2 (green) in SP cells compared with NSP cells. The second panel showed the increased expression of Oct3/4 (green) along with PD2 in SP cells compared with NSP cells. The third panel showed the membrane localisation and increased expression of β-catenin (red) along with PD2 (green) expression. DAPI was used for nuclear staining.