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. 2014 May 29;111(3):506–514. doi: 10.1038/bjc.2014.289

Figure 2.

Figure 2

CD10(+)/(–) cell viability in response to treatment with cisplatin, fluorouracil, or radiation and cell cycle analysis. (A–D) CD10(+) and CD10(−) subpopulations were isolated by FACS and cultured with 0.1–5 μM cisplatin or 0.5–50 μM fluorouracil for 72 h. Viability in response to cisplatin in FaDu (A) and Detroit562 (B) or fluorouracil in FaDu (C) and Detroit562 (D) was then measured. Statistical analysis was performed using two-way analysis of variance. (E) Alternatively, cells were exposed to radiation at single fraction 8 Gy. After 72 h, cell viability was measured. Data represent means±s.e.m.; **P<0.01. (F) Cell cycle analysis of CD10-positive and -negative subpopulations was performed after staining with Hoechst33342. (G) The cell cycle phase distribution of CD10 (+)/(–) subpopulations. **P<0.01; n.s., not significant.