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. 2014 May 29;111(3):506–514. doi: 10.1038/bjc.2014.289

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Copyright © 2014 Cancer Research UK

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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CD10 and sphere formation. (A–B) CD10 expression in sphere cells was compared with that of adherent (control) cells in FaDu and Detroit562 by flow cytometry. (C) CD10(+) and CD10(−) cells were sorted and the morphology of spheroid colonies in FaDu were examined. Representative images are shown. (D–E) The number of spheroid colonies formed in CD10(+)/(−) FaDu (D) and Detroit562 (E) were calculated. (F–G) The sizes of spherical colonies of CD10(+)/(−) in FaDu (F) and Detroit562 (G) were also determined. Data represent means±s.e.m.; *P<0.05; **P<0.01.