Figure 1.

Cisplatin induces increased PAFR expression in human ovarian cancer cells. Ovarian cancer cells were treated with different doses (0–60 μM) of cisplatin (CDDP) for 24 h (A and B, upper panels) or with a constant dose (10 μM) of CDDP for different time intervals as indicated (A and B, lower panels). Total RNA and protein were extracted and analysed for PAFR expression by real-time PCR and immunoblot analysis, respectively. 18 s rRNA (realtime PCR) and β-actin (immunoblot) were used as internal controls. The data show that CDDP induces a dose- and time-dependent expression of PAFR levels as a transcript and as a protein. Bars represent the average of the triplicates±s.d.; * (P<0.05) and ** (P<0.01) indicate a statistically significant difference compared with the untreated control.