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. 2014 Jul 21;14:89. doi: 10.1186/1471-2261-14-89

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Copyright © 2014 Sowah et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of hypertrophic stimuli on cardiomyocyte size. Cardiomyocytes isolated from the ventricles of WT (ae3^+/+)(A) and ae3^−/−(B) adult mice were subjected to vehicle alone (control), ANGII (1 μM) and PE (10 μM) treatment for 24 h, following an 18 h pre-treatment period. Images of the cardiomyocytes, taken pre- and post-treatment using a QICAM fast cooled 12-bit color camera, were quantified to measure the cell surface area. In the control group, equal volume of the vehicle was added. C, Cell surface areas were expressed as a percentage of vehicle-treated control groups (open bars) and compared to the ANGII (black bars) and PE (blue bars) treated groups. *P < 0.05, relative to control group (n = 10 mice in each group).