Figure 4.

NAPH vaccination elicits robust NY-ESO-1-specific cellular response in vitro. A, The cytotoxicity of splenocytes against NY-ESO-1-B16 cells was examined in a 4-h ⁵¹Cr-release assay. Points, mean of triplicate samples; bars, SD. B, IL-4/IFN-γ ELISPOT responses were performed at one week post-immunization (n = 3, three replicates). C, Intracellular staining of IFN-γ in CD4⁺ and CD8⁺ T cells. Similar results were obtained from three independent experiments (*P < 0.05; ** P < 0.005).